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. 2022 Feb 8;8:792322. doi: 10.3389/fvets.2021.792322

Figure 3.

Figure 3

Detection limit of conventional PCR. Templates of pMD18T-FPV (A), pMD18T-FCV-1 (B), and pMD18T-FHV-1 (C) were diluted by 10 times gradient to a dilution factor that could not be detected by conventional PCR. The detection limit was 5 × 103 copies/assay for FPV and 5 × 102 copies/assay for FCV and FHV-1. Template amount for curves 2–8 lanes was 5 × 107-5 × 101 copies/assay. M, DL600 marker; NC, negative control.