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. 2022 Feb 8;8:792322. doi: 10.3389/fvets.2021.792322

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Copyright © 2022 Cao, Tang, Zhang, Li, Li, Tian and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Amplification and standard curves of the triplex assay. Serially diluted plasmids were mixed at equal concentration (from 5 × 10⁷ copies/assay to 5 × 10¹ copies/assay) and used as template for RT-PCR. Amplification curves of the triplex assay for the detection of FPV (A), FCV (C), and FHV-1 (E). The standard curves of FPV (B), FCV (D), and FHV-1 (F) were generated by plotting the Ct values (Y-axis) against the logarithm of copy numbers of plasmids (X-axis).