Table 1.
Primers and probes.
| Namea | Sequence (5′-3′)b |
Target genes |
Amplicon
size (bp) |
|---|---|---|---|
| FPV-F | CGGGGGTGGTGGTGGTT | VP2 | 112 bp |
| FPV-R | GCTTGAGTTTGCTGTGATTTCC | ||
| FPV-P | FAM - CTGGGGGTGTGGGGATTTCTACG - BHQ1 | ||
| FCV-F | CGCCCTACACTGTGATGTG | OFR2 | 165 bp |
| FCV-R | GAGTTCTGGGTAGCAACACAT | ||
| FCV -P | VIC - ATGTGCTCAACCTGCGCTAACGTGC - BHQ-1 | ||
| FHV-1-F | ATTTGCCGCACCATACCT | TK | 140 bp |
| FHV-1-R | GCGAGTGGGAAACAGACC | ||
| FHV-1-P | Texas Red - CTTTTACATTCCAGACTATCCACAATAACAGG - BHQ-2 |
a
F, forward primer; R, reverse primer; P, TaqMan probe.
b
FAM, 6-carboxy-fluorescein; VIC, 6-carboxy-rhodamine; BHQ, black hole quencher.