Table 2.
Sensitivity of the real-time PCR.
|
Number of DNA copies (copies/assay) |
Triplex real-time PCR Ct valuea (mean ±SD) |
Uniplex real-time PCR Ct valueb (mean ±SD) |
||||
|---|---|---|---|---|---|---|
| FPV | FCV | FHV-1 | FPV | FCV | FHV-1 | |
| 5 × 107 | 14.39 ± 0.25 | 14.44 ± 0.01 | 16.17 ± 0.11 | 14.32 ± 0.25 | 14.81 ± 0.15 | 16.11 ± 0.23 |
| 5 × 106 | 18.02 ± 0.45 | 18.46 ± 0.05 | 19.62 ± 0.22 | 18.32 ± 0.05 | 18.18 ± 0.25 | 19.69 ± 0.07 |
| 5 × 105 | 21.73 ± 0.16 | 21.53 ± 0.22 | 22.47 ± 0.36 | 21.93 ± 0.02 | 21.37 ± 0.03 | 22.82 ± 0.10 |
| 5 × 104 | 25.93 ± 0.06 | 25.30 ± 0.02 | 25.98 ± 0.01 | 25.50 ± 0.66 | 24.86 ± 0.03 | 26.28 ± 0.12 |
| 5 × 103 | 29.84 ± 0.13 | 28.42 ± 0.03 | 29.40 ± 0.06 | 27.62 ± 0.16 | 28.07 ± 0.52 | 29.95 ± 0.03 |
| 5 × 102 | 32.91 ± 0.21 | 31.94 ± 0.02 | 32.61 ± 0.03 | 31.76 ± 0.35 | 31.60 ± 0.42 | 32.98 ± 0.19 |
| 5 × 101 | 35.67 ± 0.83 | 35.62 ± 0.77 | 34.93 ± 0.53 | 34.08 ± 0.33 | 33.41 ± 0.08 | 35.25 ± 0.16 |
a
The result was considered positive if mean Ct value ≤ 36.
b
Each reaction was performed in triplicate, and the results are shown as the mean ± SD.