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. 2022 Feb 8;8:792322. doi: 10.3389/fvets.2021.792322

Table 3.

Intra- and inter-assay reproducibility of the triplex assay.

Name Number of DNA
copies
(copies/assay)
Intra-assay a Inter-assay a
Mean SD CV (%) Mean SD CV (%)
FPV 5 × 107 14.29 0.15 1.04 14.25 0.21 1.47
5 × 105 21.66 0.28 1.29 21.98 0.41 1.87
5 × 103 29.92 0.29 0.96 30.10 0.28 0.93
FCV 5 × 107 14.52 0.10 0.69 14.72 0.40 2.72
5 × 105 21.42 0.25 1.17 21.28 0.34 1.60
5 × 103 28.35 0.35 1.23 28.89 0.67 2.32
FHV-1 5 × 107 16.24 0.11 0.68 16.44 0.37 2.25
5 × 105 22.53 0.42 1.86 22.58 0.72 3.19
5 × 103 29.34 0.21 0.72 29.58 0.25 0.85