Table 4.
Detection of the co-infection models by triplex real-time PCR.
| Co-infection proportiona |
Virus titer (TCID50/assay) |
Co-infection real-time PCR Ct value (mean ±SD) |
||||
|---|---|---|---|---|---|---|
| FPV | FCV | FHV-1 | FPV | FCV | FHV-1 | |
| FPV:FCV:FHV-1 = 10:1:1 | 106 | 105 | 105 | 9.35 ± 0.11 | 33.72 ± 0.43 | 22.87 ± 0.37 |
| FPV:FCV:FHV-1 = 1:10:1 | 105 | 106 | 105 | 14.09 ± 0.88 | 29.56 ± 0.52 | 22.88 ± 0.03 |
| FPV:FCV:FHV-1 = 1:1:10 | 105 | 105 | 106 | 13.59 ± 0.71 | 33.88 ± 0.28 | 19.77 ± 0.56 |
| FPV:FCV = 1:1 | 105 | 105 | – | 14.40 ± 0.47 | 33.10 ± 0.16 | – |
| FPV:FCV = 10:1 | 106 | 105 | – | 9.69 ± 0.21 | 34.02 ± 0.12 | – |
| FPV:FHV-1 = 1:1 | 105 | – | 105 | 13.97 ± 0.97 | – | 22.97 ± 0.16 |
| FPV:FHV-1 = 10:1 | 106 | – | 105 | 9.46 ± 0.24 | – | 22.30 ± 0.82 |
| FCV:FHV-1 = 1:1 | – | 105 | 105 | – | 33.49 ± 0.39 | 22.85 ± 0.65 |
| FCV:FHV-1 = 10:1 | – | 106 | 105 | – | 29.52 ± 0.59 | 22.89 ± 1.06 |
a
Viruses of different titer were mixed in corresponding proportions and used as templates for real-time PCR.