Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 8;16:794653. doi: 10.3389/fnins.2022.794653

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Leggieri, García-González, Torres-Perez, Havelange, Hosseinian, Mech, Keatinge, Busch-Nentwich and Brennan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

CRISPR-Cas9 generated a 27 bp insertion that disrupts the ankk1 sequence (A) Comparison of wild type (top) and mutant (bottom) ankk1 sequences. crRNA is highlighted in blue. PAM sequence is highlighted in yellow. The restriction site (that is disrupted in the F₀ screening) appears in red, underlined. (B) Comparison of wild type (top) and mutant (bottom) amino acid sequences. Mutant sequence generates an early stop codon.