FIGURE 4.

MSCs of different origin induce microvascular network formation in ECs with significantly different vasculature area, vessel diameter, and vasculature length. The presence of MSCs greatly improves microvasculature parameters compared to ECs cultured alone. (A) Representative images of microvascular networks derived from EC-BMSC and EC-ASC co-cultures and EC alone at day 6 and corresponding binary images. Scale bars, 200 µm. (B) Morphological parameters (vasculature area, average vessel diameter, and vasculature length) of MSCs supported microvascular networks are shown individually for BMSCs and ASCs derived from different donors. Data are presented as means of 9 ROIs (3 devices, 3 ROIs per device). Comparison between BMSCs and ASCs as well as comparison of BMSCs and ASCs to EC alone was performed on mean values from EC-BMSC and EC-ASC co-cultures (3 donors, 3 devices per donor, 3 ROIs per device). ***—p < 0.001 with Unpaired student’s t-test. p values were corrected to control the false discovery rate. Donor cell lines BMSC 2 and ASC 1 were used for generation of data presented in (A).