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. 2022 Feb 8;10:764237. doi: 10.3389/fbioe.2022.764237

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Copyright © 2022 Mykuliak, Yrjänäinen, Mäki, Gebraad, Lampela, Kääriäinen, Pakarinen, Kallio, Miettinen and Vuorenpää.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Measured average flow rates (Q) [(90 µl + 90 µl)+(50 µl + 50 µl)] on two independent biological replicates (N = 2) on (A) day 0, (B) day 2, and (C) day 4 for both EC-ASC (*) and EC-BMSC (□) co-cultures. (C) At day 4, there is minimal flow front movement between two consecutive image indexes due to decreased flow rate. This results in reduced accuracy of the used analysis method that is seen as oscillation of the measured flow rate values. Donor cell lines BMSC 1 and ASC 3 were used for the flow characterization co-cultures.