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. 2022 Feb 8;10:764237. doi: 10.3389/fbioe.2022.764237

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Copyright © 2022 Mykuliak, Yrjänäinen, Mäki, Gebraad, Lampela, Kääriäinen, Pakarinen, Kallio, Miettinen and Vuorenpää.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitative RT-PCR analysis of gene expression in ASC- and BMSC-supported microvascular networks. Heatmap shows relative mRNA expression of pericyte-specific (PC) and endothelial-specific (EC) genes, ECM protein (ECM), angiogenic growth factors (GF), and other genes involved in vasculature morphogenesis and stability in EC-ASC and EC-BMSC co-cultures after 6 days of culture. Gene expression fold change of EC-BMSC and EC-ASC cocultures was computed relative to the average expression in EC-ASC co-cultures. The reference genes were 18S and GAPDH (for PECAM1, VEGFA, PDGFRB, CSPG2, ACTA2, ANGPT1, FGF2) and PECAM1 (for VWF, CDH5, KDR, FLT1, ANGPT2).