FIGURE 3.

Evaluation of different dissociation formats using cell aggregates. MCF-7 cells were passed through the channel module 10 times and filter module once (10C, 1F), both channel and filter modules 10 times (10C + F), or the filter module 10 times (10F) at different flow rates. Results are shown for (A) total single cell, (B) aggregate, and (C) live single cell yeilds, which are all normalized to the unprocessed control. Also presented is (D) single cell viability. Optimal results were observed at 40 ml/min using multiple filter module passes. Data are presented as mean values ± SEM from at least three independent experiments. Two-sided t test was used for statistical testing. Stars indicate p < .05 and double stars indicate p < .01 relative to the unprocessed control.