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PC12 proliferation was tested after the cultured medium was added to 5 µg/ml, 10 µg/ml, and 20 µg/ml exosomes. Untreated PC12 cells served as the Control group. Expression levels of miR-218 targeted genes (Robo 1, Robo 2, Sfrp 2, and Dkk 2) were examined by qRT-PCR in untreated PC12 cells (Control group), PC12 cells of transfected miR-218 plasmid, and two exosomes groups.
