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. 2022 Feb 8;13:840995. doi: 10.3389/fphys.2022.840995

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Copyright © 2022 Tzounakas, Anastasiadi, Lekka, Papageorgiou, Stamoulis, Papassideri, Kriebardis and Antonelou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Correlation between vesicle-enclosed Hb and storage hemolysis in blood bank conditions. (A) Scatter plots showing the correlation between total free and vesicle-enclosed Hb in whole blood, CPDA and CPD-SAGM units, from mid-storage onward. (B) Threshold analysis in CPDA blood units. The dashed boxes indicate the hemolysis or EV-Hb percentage (insert) values that serve as cutoffs. (C) Dynamic light scattering (DLS) analysis in selected CPDA samples of high and low hemolysis (above and below the threshold, respectively; n = 5 per group) during middle and late storage (horizontal axis). *p < 0.05, high vs low storage hemolysis.