Fig. 6.

Angle probability distribution of Trp246 and Tyr288. A. $\chi$₂ of Trp246^6.48 is presented for antagonist-bound A_2AR in the absence and presence of the linker, in black and red, respectively. The corresponding angle is also measured in crystal structure of antagonist-bound A_2AR (PDB ID:44EIY) and indicated with dashed orange line. B. The same as in A but for $\chi$₂ of Tyr288^7.53. Comparison of orientation of C. Trp246^6.48 in the absence (green) and presence (orange) of the bivalent ligand. Oreintations of Tyr288^7.53 in the D absence of linker (dark and light green) and E. presence of linker (orange). The orientation of Trp^6.48 and Tyr^7.53 in the crystal structure of antagonist-bound receptor is shown in pink. The residues are shown on the 3D structure of antagonist bound A_2AR. Timeline plots of Trp246^6.48 and Tyr288^7.53 of both replicates are given in the SI Fig. 6.