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. 2022 Feb 5;25(3):103878. doi: 10.1016/j.isci.2022.103878

Figure 4.

Figure 4

The VLE RNA transport granules behave as phase-separated biocondensate

(A) Oocytes were taken 16 h after injection with Alexa Fluor 546-labeled VLE RNA and treated with 5% 1,6-hexanediol for 60 min and then fixed.

(B) Depolymerization of microtubules with nocodazole. Oocytes were incubated with DMSO (vehicle control) or nocodazole (5 μg/mL) for 6 h, fixed and bisected prior to immunohistochemical staining using anti-β-tubulin/Alex Fluor 546-conjugated anti-rabbit antibodies.

(C) Following injection with Alexa Fluor 546-labeled VLE RNA, oocytes were placed in culture medium containing DMSO or nocodazole for 48 h and then fixed. Scale bars = 50 μm unless noted otherwise.