Antibodies |
|
Rabbit Polyclonal anti-β-tubulin |
Novus |
NB600-936; RRID:AB_10000656
|
Rabbit polyclonal anti-GFP |
Invitrogen |
PA1-980A; RRID:AB_325960
|
|
Chemicals, peptides, and recombinant proteins |
|
Liberase TH collagenase |
Sigma Aldrich |
5401135001 |
Antimycin A |
Sigma Aldrich |
A8674 |
RNasin |
Promega |
N2511 |
T7 RNA polymerase |
Promega |
P2075 |
SP6 RNA polymerase |
Promega |
P1085 |
Ribo m7G Cap Analog |
Promega |
P1711 |
Oligomycin A |
Sigma Aldrich |
75351 |
Nocodazole |
Sigma Aldrich |
M1404 |
Leibovitz L-15 medium |
Sigma Aldrich |
L1518 |
Insulin |
Sigma Aldrich |
I0516 |
Penicillin G |
Sigma Aldrich |
P3032 |
Streptomycin |
Sigma Aldrich |
S9137 |
L-glutamine |
Fisher Biotech |
BP379 |
Nystatin |
Sigma Aldrich |
N1638 |
Gentamycin |
Dot Scientific |
DSG38000 |
Biotin-GMP |
Tri-Link BioTechnologies |
N-6003 |
Streptavidin-agarose beads |
Sigma Aldrich |
S1638 |
Alexa Fluor 546-14-UTP |
Thermo Fisher |
C11404 |
Alexa Fluor 488-5-UTP |
Thermo Fisher |
C11403 |
adenosine 5'-(β,γ-imido) triphosphate |
Sigma Aldrich |
A2647 |
1,6-hexanediol |
Sigma Aldrich |
240117 |
Vitellogenin |
This paper |
N/A |
S-Trap columns |
Protifi |
CO2-Mini-80 |
Oasis HLB Vac Cartridge |
Waters |
186000383 |
|
Critical commercial assays |
|
Primescript Master Mix |
Promega |
A5001 |
|
Experimental models: Organisms/strains |
|
Xenopus laevis: eGFP-OMP25 |
Marine Biological Lab |
NXR_0059 |
Xenopus laevis: Albino |
Nasco |
LM00531(B) |
Xenopus laevis: Wild type |
Nasco |
LM00531 |
|
Recombinant DNA |
|
Plasmid: pBSVg1-B |
This paper |
N/A |
ATeam 1.03-nD/nA/pcDNA3 |
Kotera et al., 2010 |
Addgene #51958; RRID:Addgene_51958 |
|
Deposited data |
|
MS/MS data |
MassIVE |
ftp://massive.ucsd.edu/MSV000087737 |
|
Software and algorithms |
|
ImageJ |
Schneider et al., 2012 |
https://imagej.nih.gov/ij/ |