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. 2022 Feb 5;25(3):103878. doi: 10.1016/j.isci.2022.103878
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Rabbit Polyclonal anti-β-tubulin Novus NB600-936; RRID:AB_10000656
Rabbit polyclonal anti-GFP Invitrogen PA1-980A; RRID:AB_325960

Chemicals, peptides, and recombinant proteins

Liberase TH collagenase Sigma Aldrich 5401135001
Antimycin A Sigma Aldrich A8674
RNasin Promega N2511
T7 RNA polymerase Promega P2075
SP6 RNA polymerase Promega P1085
Ribo m7G Cap Analog Promega P1711
Oligomycin A Sigma Aldrich 75351
Nocodazole Sigma Aldrich M1404
Leibovitz L-15 medium Sigma Aldrich L1518
Insulin Sigma Aldrich I0516
Penicillin G Sigma Aldrich P3032
Streptomycin Sigma Aldrich S9137
L-glutamine Fisher Biotech BP379
Nystatin Sigma Aldrich N1638
Gentamycin Dot Scientific DSG38000
Biotin-GMP Tri-Link BioTechnologies N-6003
Streptavidin-agarose beads Sigma Aldrich S1638
Alexa Fluor 546-14-UTP Thermo Fisher C11404
Alexa Fluor 488-5-UTP Thermo Fisher C11403
adenosine 5'-(β,γ-imido) triphosphate Sigma Aldrich A2647
1,6-hexanediol Sigma Aldrich 240117
Vitellogenin This paper N/A
S-Trap columns Protifi CO2-Mini-80
Oasis HLB Vac Cartridge Waters 186000383

Critical commercial assays

Primescript Master Mix Promega A5001

Experimental models: Organisms/strains

Xenopus laevis: eGFP-OMP25 Marine Biological Lab NXR_0059
Xenopus laevis: Albino Nasco LM00531(B)
Xenopus laevis: Wild type Nasco LM00531

Recombinant DNA

Plasmid: pBSVg1-B This paper N/A
ATeam 1.03-nD/nA/pcDNA3 Kotera et al., 2010 Addgene #51958; RRID:Addgene_51958

Deposited data

MS/MS data MassIVE ftp://massive.ucsd.edu/MSV000087737

Software and algorithms

ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/