Drugs for respiratory chain manipulation

Reagent	Final concentration	Amount
ADP (MW: 501.32)	1 mM	501.32 mg ADP in 10 mL MAS buffer (100 mM).
Pyruvate/malate (MW: 88.06/134.09)	5 mM/2.5 mM	440.3 mg pyruvate + 335.2 mg malate in 10 mL MAS buffer (500 mM/250 mM).
Rotenone (MW: 394.42)∗	1 μM	5 mg rotenone in 1.267 mL DMSO (10 mM). Working solution (concentration 225 μM): 22.5 μL of the stock solution diluted in 977.5 μL MAS-BSA.
Succinate (MW: 118.09)	10 mM	590 mg succinic acid to 10 mL MAS buffer (500 mM).
Antimycin A (MW: 534.60)∗	20 μM	25 mg Antimycin A in 1.27 mL DMSO (36 mM). Working stock solution (concentration 2 mM): 16.7 μL of the stock solution diluted in 283.3 μL MAS-BSA).
FCCP (MW: 254.17)∗	1 μM	10 mg FCCP in 787 μL DMSO (concentration: 50 mM). Working solution (concentration: 100 μM): add 1 μL of the stock solution to 499 μL MAS buffer.
Oxaloacetate (OAA; MW: 132.07)		528.28 mg OAA in 10 mL MAS buffer (concentration: 400 mM).

Adjust pH of the drugs to 7.2 with KOH. Stock solutions may be stored as aliquots for up to 2 months at –20°C (in the dark since some compounds may be light-sensitive).