Surface markers labeling solution

Reagent	Final concentration	Amount
FACS buffer	-	49.5 μL
CD11b-APC	1:200	0.25 μL per sample
CD45-PE-Cy7	1:200	0.25 μL per sample
Total		50 μL

FACS staining solution is made fresh each time and should be kept at 4°C in the dark.