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MAS buffer (1×)
| Reagent | Final concentration | Amount |
|---|---|---|
| Mannitol (MW: 182.17 g/mol) | 220 mM | 40.07 g |
| Sucrose (MW: 342.30 g/mol) | 70 mM | 23.96 g |
| KH2PO4 (MW: 136.09 g/mol) | 10 mM | 1.36 g |
| MgCl2 1 M solution | 5 mM | 5 mL |
| HEPES 1 M solution | 2 mM | 2 mL |
| EGTA 0.25 M solution | 1 mM | 4 mL |
| ddH2O | - | 989 mL |
| Total | 1 L |
Adjust the pH to 7.2 with 0.1 M KOH. After filter-sterilization, this buffer may be stored at 4°C for up to 2 months.
