Permeabilization solution

Reagent	Final concentration	Amount
MAS-BSA buffer	-	5935.8 μL
Plasma Membrane Permeabilizer (PMP)	1 nM	1.2 μL
ADP	1 mM	60 μL
TMRM (10 mM)	50 nM	3.0 μL
Total		6,000 μL

Permeabilization solution should be prepared freshly prior to the experiment at 4°C in the dark