Figure 1.

Screening of chalcone analogs for therapeutic activity in prostate cancer

(A) Structures of 22 chalcone analogs used for screening against the NCI-60 panel of 60 cancer cell lines.

(B) Heatmap of growth inhibition of DU145 and PC3 prostate cancer cell lines from the NCI-60 cell-line panel upon treatment with the indicated chalcones shown in Figure S1 and Table S1. Each cell line was treated with the indicated chalcone for 48 h at a concentration of 10 μM. Growth inhibition was measured using sulforhodamine B assay per standard NCI protocol. Growth inhibition is indicated in a gradient from blue (no growth inhibition) to red (complete growth inhibition).

(C) Chemical structure of SU086.

(D) IC₅₀ determination by cell titer blue viability assay in the indicated prostate cancer cells treated for 72 h with the indicated concentrations of SU086. Calculated IC₅₀ is calculated in micromolars. Error bars indicate ±SD.