Reagent or resource	Source	Identifier
Antibodies
Anti-Human-PGK1	Santa Cruz Biotechnology	Cat#sc-130335
Anti-Human-Ki67	Santa Cruz Biotechnology	Cat#sc-23900
Anti-Human-AR/Androgen Receptor Antibody	Santa Cruz Biotechnology	Cat#sc-7305
Anti-Human-α-actin (alpha-SM1)	Santa Cruz Biotechnology	Cat#sc-130617
Anti-Human-cleaved Caspase-3 (Asp175)	Cell Signaling Technology	Cat#9661
Anti-Human-vimentin (D21H3)	Cell Signaling Technology	Cat#5741
Anti-Human-E-cadherin (24E10)	Cell Signaling Technology	Cat#3195
Anti-Human-HSP90 (C45G5)	Cell Signaling Technology	Cat#4877
Anti-Human-HSP27/HSPB1 (A0240)	ABclonal	Cat#A0240
Anti-Human-YWHAZ Rabbit	ABclonal	Cat#A13370
Anti-Human-HSP90-alpha (D1A7)	ABclonal	Cat#8165
Anti-Human-HSP90-beta	Abcam	Cat#ab32568
Biological samples
Radical prostatectomy specimens	Stanford University Department of Urology	N/A
Chemicals, peptides, and recombinant proteins
R1881	Sigma Aldrich	Cat#R0908
ROK inhibitor Y-27632	Selleck Chemicals	Cat#S1049
SU086	This paper	N/A
Enzalutamide	TargetMol	Cat#T6002
Abiraterone acetate	TargetMol	Cat#T6215
Testosterone	Sigma Aldrich	Cat#58-22-0
Critical commercial assays
CellTiter-Blue Cell Viability Assay	Promega	Cat#G8081
Corning BioCoat Matrigel Invasion Chamber - Matrigel	Fisher Scientific	Cat#08-774-122
Costar Transwell Permeable Supports	Fisher Scientific	Cat#07-200-150
Cellular Thermal Shift Assay	Savitski et al.27	N/A
Seahorse XFp glycolysis stress test kit	Agilent Technologies	Cat#103017-100
Deposited data
Proteomics	PRIDE	PXD030524
Cellular Thermal Shift Assay (CETSA)	PRIDE	PXD030524
Experimental models: Cell lines
22RV1	ATCC	CRL-2505
DU145	ATCC	HTB-81
C4-2	ATCC	CRL-3314
PC3	ATCC	CRL-1435
LuCaP 136	Valta et al.23	N/A
LuCaP 147	Saar et al.22	N/A
Experimental models: Organisms/strains
BALB/c male mice	Charles River Laboratories	N/A
NOD-SCID-IL2Rγ–null	Jackson Laboratory	N/A
Oligonucleotides
hHSP90AA1[shRNA#1] AGCTGCATATTAACCTTATAC	VectorBuilder Inc.	N/A
Scramble[shRNA#1] CCTAAGGTTAAGTCGCCCTCG	VectorBuilder Inc.	N/A
Software and algorithms
Prism (version 9.2)	Graphpad	https://www.graphpad.com/scientific-software/prism/
ImageJ	ImageJ	https://imagej.net/Downloads
Byonic 2.11.0	Protein Metrics	N/A
Other
ForteBio RED384 Label-Free Detection Systems	Sartorius	N/A