Antibodies |
|
Mouse monoclonal anti-CD56 BUV563 (clone NCAM16.2) |
BD BioSciences |
Cat# 565704, Lot# 8250989; RRID: AB_2744431
|
Mouse monoclonal anti-CD16 BUV496 (clone 3G8) |
BD BioSciences |
Cat# 564653, lot# 8291900; RRID: AB_2744294
|
Mouse monoclonal anti-NKp44 AlexaFluor647 (clone p44-8) |
BD BioSciences |
Cat# 558564, lot# 5082973; RRID: AB_647153
|
Mouse monoclonal anti-CD25 PE-CF594 (clone M-A251) |
BD BioSciences |
Cat# 562403, lot# 9099748; RRID: AB_11151919
|
Mouse monoclonal anti-PD1 BB700 (clone EH12.1) |
BD BioSciences |
Cat# 566460, Lot# 9182916, 8346689; RRID: AB_2744348
|
Mouse monoclonal anti-HLA-DR BV650 (clone G46-6) |
BD BioSciences |
Cat# 564231, lot# 9017957; RRID: AB_2738685
|
Mouse monoclonal anti-TIM-3 BV711 (clone 7D3) |
BD BioSciences |
Cat# 565566, lot# 9049619, 9116947; RRID: AB_2744370
|
Mouse monoclonal anti-CD38 BV738 (clone HIT2) |
BD BioSciences |
Cat# 563964, lot# 7338505, 9151925; RRID: AB_2738515
|
Mouse monoclonal anti-DNAM-1 BUV395 (clone DX11) |
BD BioSciences |
Cat# 742498, lot# 9136597, 9276805, 9276799, 9276800; RRID: AB_2740831
|
Mouse monoclonal anti-CXCR4 BUV737 (clone 12G5) |
BD BioSciences |
Cat# 741862, lot# 9276807, 9276809, 9276810; RRID: AB_2871192
|
Mouse monoclonal anti-Ki67 AlexaFluor700 (clone B56) |
BD BioSciences |
Cat# 561277, lot# 7349946; RRID: AB_10611571
|
Mouse monoclonal anti-perforin 1 BB755-P (clone δG9) |
BD BioSciences |
Custom conjugate, Cat# 624391, lot# 9101891 |
Mouse monoclonal anti-granzyme B BB790-P (clone GB11) |
BD BioSciences |
Custom conjugate, Cat# 624296, lot# 9130980 |
Mouse monoclonal anti-CD19 V500 (clone HIB19) |
BD BioSciences |
Cat# 561121, lot# 7066974; RRID: AB_10562391
|
Mouse monoclonal anti-CD14 V500 (clone MΦP9) |
BD BioSciences |
Cat# 562693, lot# 8215846; RRID: AB_2737727
|
Mouse monoclonal anti-CD3 PE-Cy5 (clone HIT3a) |
BioLegend |
Cat# 300310, lot# B181404, B291468; RRID: AB_314046
|
Mouse monoclonal anti-NKG2D BV421 (clone 1D11) |
BioLegend |
Cat# 320821, lot# B274633; RRID: AB_2566510
|
Mouse monoclonal anti-2B4 APC-Cy7 (clone C1.7) |
BioLegend |
Cat# 329518, lot# B329518, B219470, B276619; RRID: AB_2572015
|
Mouse monoclonal anti-CD319 PE (clone 162.1) |
BioLegend |
Cat# 331806, lot# B268818; RRID: AB_2239190
|
Mouse monoclonal anti-LAG-3 PE-Cy7 (11C3C65) |
BioLegend |
Cat# 369310, lot# B289010; RRID: AB_2629753
|
Mouse monoclonal anti-TIGIT BV605 (clone A15153G) |
BioLegend |
Cat# 372711, lot# B240084, B281575; RRID: AB_2632926
|
Mouse monoclonal anti-NKG2A VioBright FITC (clone REA110) |
Miltenyi Biotec |
Cat# 130-105-646, lot# 5160809205; RRID: AB_2655382
|
Orthoclone OKT3 (MACS GMP CD3 pure) |
Miltenyi Biotech |
Cat# 170-076-124 |
|
Biological samples |
|
Human AB serum |
Lonza |
Cat# 14-490E; current Cat# 4W-320 |
|
Chemicals, peptides, and recombinant proteins |
|
CellGro SCGM serum-free cell culture media |
CellGenix |
Cat# 0020902-0500 |
IL-2 (Proleukin) |
Chiron, currently Clinigen |
https://www.medicines.org.uk/emc/product/291/smpc |
Pluronic F68 |
Life Technologies |
Cat# 24040032 |
|
Critical commercial assays |
|
eBioscience Foxp3/Transcription Factor Staining Buffer Set |
Thermo Fisher Scientific |
Cat# 00-5523-00 |
LIVE/DEAD Fixable Aqua Dead Cell Stain Kit |
Thermo Fisher Scientific |
Cat# L34957
|
LymphoTrack Dx IGH FR1/FR2/FR3 Assay Kit A -MiSeq |
Invivoscribe |
Cat# 9-121-0129 |
QIAamp DNA micro kit |
Qiagen |
Cat# 56304 |
QIAamp DNA Blood Midi Kit |
Qiagen |
Cat# 51183 |
Agencourt AMPure XP beads |
Beckman Coulter |
Cat# A63880 |
Qubit dsDNA HS Assay Kit |
Invitrogen |
Cat# Q32854
|
Agilent High Sensitivity DNA ScreenTape Assay |
Agilent |
Cat# 5067-5584 |
Olink Immuno-Oncology panel v.3101 |
Olink Bioscience |
Cat# 95310 |
PeliKine Compact human GRANZYME B ELISA Kit |
Sanquin CLB |
Ref# M1936, no longer available |
|
Software and algorithms |
|
FlowJo v.10 |
Treestar inc. |
RRID: SCR_008520
|
GraphPad Prism v.8 |
GraphPad Software |
RRID: SCR_002798
|
LymphoTrack Dx Software – MiSeq Version 2.4.3 |
Invivoscribe |
Cat# 95000009 |
LymphoTrack MRD Software v1.2.0 |
Invivoscribe |
Cat# 75000008 |
|
Other |
|
WAVE Bioreactor™ System 2/10 |
GE Healthcare |
|
Cobas 8000 system |
Roche Diagnostics |
https://diagnostics.roche.com/global/en/products/systems/cobas_-8000-modular-analyzer-series.html#productInfo |
Beckman Coulter IMMAGE 800 Protein Chemistry Analyzer |
Beckman Coulter |
RRID: SCR_019642
|
BN ProSpec |
Siemens Healthcare GmbH |
https://www.siemens-healthineers.com/se/plasma-protein/systems/bn-prospec-system |
Atellica NEPH 630 system |
Siemens Healthcare GmbH |
https://www.siemens-healthineers.com/se/plasma-protein/systems/atellica-neph-630-system |
Sebia Hydrasys LC system |
Sébia |
https://www.sebia.com/instruments/hydrasys-2-scan-focusing/ |