Skip to main content
. 2022 Jan 28;3(2):100508. doi: 10.1016/j.xcrm.2022.100508
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Mouse monoclonal anti-CD56 BUV563 (clone NCAM16.2) BD BioSciences Cat# 565704, Lot# 8250989; RRID: AB_2744431
Mouse monoclonal anti-CD16 BUV496 (clone 3G8) BD BioSciences Cat# 564653, lot# 8291900; RRID: AB_2744294
Mouse monoclonal anti-NKp44 AlexaFluor647 (clone p44-8) BD BioSciences Cat# 558564, lot# 5082973; RRID: AB_647153
Mouse monoclonal anti-CD25 PE-CF594 (clone M-A251) BD BioSciences Cat# 562403, lot# 9099748; RRID: AB_11151919
Mouse monoclonal anti-PD1 BB700 (clone EH12.1) BD BioSciences Cat# 566460, Lot# 9182916, 8346689; RRID: AB_2744348
Mouse monoclonal anti-HLA-DR BV650 (clone G46-6) BD BioSciences Cat# 564231, lot# 9017957; RRID: AB_2738685
Mouse monoclonal anti-TIM-3 BV711 (clone 7D3) BD BioSciences Cat# 565566, lot# 9049619, 9116947; RRID: AB_2744370
Mouse monoclonal anti-CD38 BV738 (clone HIT2) BD BioSciences Cat# 563964, lot# 7338505, 9151925; RRID: AB_2738515
Mouse monoclonal anti-DNAM-1 BUV395 (clone DX11) BD BioSciences Cat# 742498, lot# 9136597, 9276805, 9276799, 9276800; RRID: AB_2740831
Mouse monoclonal anti-CXCR4 BUV737 (clone 12G5) BD BioSciences Cat# 741862, lot# 9276807, 9276809, 9276810; RRID: AB_2871192
Mouse monoclonal anti-Ki67 AlexaFluor700 (clone B56) BD BioSciences Cat# 561277, lot# 7349946; RRID: AB_10611571
Mouse monoclonal anti-perforin 1 BB755-P (clone δG9) BD BioSciences Custom conjugate, Cat# 624391, lot# 9101891
Mouse monoclonal anti-granzyme B BB790-P (clone GB11) BD BioSciences Custom conjugate, Cat# 624296, lot# 9130980
Mouse monoclonal anti-CD19 V500 (clone HIB19) BD BioSciences Cat# 561121, lot# 7066974; RRID: AB_10562391
Mouse monoclonal anti-CD14 V500 (clone MΦP9) BD BioSciences Cat# 562693, lot# 8215846; RRID: AB_2737727
Mouse monoclonal anti-CD3 PE-Cy5 (clone HIT3a) BioLegend Cat# 300310, lot# B181404, B291468; RRID: AB_314046
Mouse monoclonal anti-NKG2D BV421 (clone 1D11) BioLegend Cat# 320821, lot# B274633; RRID: AB_2566510
Mouse monoclonal anti-2B4 APC-Cy7 (clone C1.7) BioLegend Cat# 329518, lot# B329518, B219470, B276619; RRID: AB_2572015
Mouse monoclonal anti-CD319 PE (clone 162.1) BioLegend Cat# 331806, lot# B268818; RRID: AB_2239190
Mouse monoclonal anti-LAG-3 PE-Cy7 (11C3C65) BioLegend Cat# 369310, lot# B289010; RRID: AB_2629753
Mouse monoclonal anti-TIGIT BV605 (clone A15153G) BioLegend Cat# 372711, lot# B240084, B281575; RRID: AB_2632926
Mouse monoclonal anti-NKG2A VioBright FITC (clone REA110) Miltenyi Biotec Cat# 130-105-646, lot# 5160809205; RRID: AB_2655382
Orthoclone OKT3 (MACS GMP CD3 pure) Miltenyi Biotech Cat# 170-076-124

Biological samples

Human AB serum Lonza Cat# 14-490E; current Cat# 4W-320

Chemicals, peptides, and recombinant proteins

CellGro SCGM serum-free cell culture media CellGenix Cat# 0020902-0500
IL-2 (Proleukin) Chiron, currently Clinigen https://www.medicines.org.uk/emc/product/291/smpc
Pluronic F68 Life Technologies Cat# 24040032

Critical commercial assays

eBioscience Foxp3/Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00
LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific Cat# L34957
LymphoTrack Dx IGH FR1/FR2/FR3 Assay Kit A -MiSeq Invivoscribe Cat# 9-121-0129
QIAamp DNA micro kit Qiagen Cat# 56304
QIAamp DNA Blood Midi Kit Qiagen Cat# 51183
Agencourt AMPure XP beads Beckman Coulter Cat# A63880
Qubit dsDNA HS Assay Kit Invitrogen Cat# Q32854
Agilent High Sensitivity DNA ScreenTape Assay Agilent Cat# 5067-5584
Olink Immuno-Oncology panel v.3101 Olink Bioscience Cat# 95310
PeliKine Compact human GRANZYME B ELISA Kit Sanquin CLB Ref# M1936, no longer available

Software and algorithms

FlowJo v.10 Treestar inc. RRID: SCR_008520
GraphPad Prism v.8 GraphPad Software RRID: SCR_002798
LymphoTrack Dx Software – MiSeq Version 2.4.3 Invivoscribe Cat# 95000009
LymphoTrack MRD Software v1.2.0 Invivoscribe Cat# 75000008

Other

WAVE Bioreactor™ System 2/10 GE Healthcare
Cobas 8000 system Roche Diagnostics https://diagnostics.roche.com/global/en/products/systems/cobas_-8000-modular-analyzer-series.html#productInfo
Beckman Coulter IMMAGE 800 Protein Chemistry Analyzer Beckman Coulter RRID: SCR_019642
BN ProSpec Siemens Healthcare GmbH https://www.siemens-healthineers.com/se/plasma-protein/systems/bn-prospec-system
Atellica NEPH 630 system Siemens Healthcare GmbH https://www.siemens-healthineers.com/se/plasma-protein/systems/atellica-neph-630-system
Sebia Hydrasys LC system Sébia https://www.sebia.com/instruments/hydrasys-2-scan-focusing/