Table 2.
Primer sequence for qPCR and corresponding probe number for genes of interest from Roche Universal Probe Library (UPL®) for adipose and brain tissue samples.
| Gene: name | Primer sequence | UPL probe | |
|---|---|---|---|
| PLIN1: perilipin | Forward | AGGATGGCAGTCAACAAAGG | 42 |
| Reverse | GCAGCACATTCTCCTGCTC | ||
| ACAB: acetyl-CoA carboxylase | Forward | CAGACGCTACAGGTCCCAAC | 37 |
| Reverse | CTGTCCACTCCACTGTCAGG | ||
| FAS: Fas cell surface death receptor | Forward | CAGGCACACACGATGGAC | 11 |
| Reverse | CGGAGTGAATCTGGGTTGAT | ||
| ADIPOQ: adiponectin | Forward | GGTGAGAAGGGTGAGAAAGGA | 85 |
| Reverse | TTCACCGATGTCTCCCTTAG | ||
| PNPLA2: adipose triglyceride lipase | Forward | CTCCACCAACATCCACGAG | 89 |
| Reverse | CCCTGCTTGCACATCTCTC | ||
| PER1: period circadian | Forward | CTCTTCCACAGCTCCCTCA | 87 |
| Reverse | CTTTGGATCGGCAGTGGT | ||
| LPL: lipoprotein lipase | Forward | ATGTGGCCCGGTTTATCA | 25 |
| Reverse | CTGTATCCCAAGAGATGGACATT | ||
| PCK1: phosphoenolpyruvate carboxykinase | Forward | CGAAAGCTCCCCAAGTACAA | 20 |
| Reverse | GCTCTCTACTCGTGCCACATC | ||
| ABHD5: abhydrolase domain containing 5 | Forward | GGACAAAATGATCTTGCTTGG | 66 |
| Reverse | CCCAAGGCTCCACTAAAATG | ||
| NR3C1: nuclear receptor subfamily 3, group C, member 1 (α glucocorticoid receptor) | Forward | TTTTCTTCAAAAGAGCAGTGGA | 11 |
| Reverse | GCATGCTGGGCAGTTTTT | ||
| NR3C2: nuclear receptor subfamily 3, group C, member 2 (mineralocorticoid receptor) | Forward | CATCATGAAAGTTTTGCTGCTACT | 64 |
| Reverse | TCTTTGATGTAATTTGTCCTCATTTC | ||
| SGK1: serum and glucocorticoid-regulated kinase 1 | Forward | GACAGGACTGTGGACTGGTG | 24 |
| Reverse | TTTCAGCTGTGTTTCGGCTA | ||
| FKBP5: FK506 binding protein 5 | Forward | GGATATACGCCAACATGTTCAA | 15 |
| Reverse | CCATTGCTTTATTGGCCTCT | ||
| LIPE: hormone sensitive lipase | Forward | GGAAGTGCTATCGTCTCTGG | SYBR® Green master mix |
| Reverse | GGCAGTCAGTGGCATCTC | ||
| ABCB1: ATP binding cassette subfamily B member 1 | Forward | AAGGCATTTACTTCAAACTTGTCA | 18 |
| Reverse | TGGATTCATCAGCTGCATTTT | ||
| ABCC1: ATP binding cassette subfamily C member 1 | Forward | GCCTATTACCCCAGCATCG | 28 |
| Reverse | GATGCAGTTGCCCACACA | ||
| MT1: metallothionein 1 | Taqman Gene expression assay Hs04401199_s1 (Thermo Fisher Scientific) |
||
| Housekeeping genes | |||
| RNA18S: ribosomal RNA 18s | Forward | CTTCCACAGGAGGCCTACAC | 46 |
| Reverse | CGCAAAATATGCTGGAACTTT | ||
| PPIA: peptidylpropyl isomerase A (cyclophilin A) | Forward | ATGCTGGACCCAACACAAAT | 48 |
| Reverse | TCTTTCACTTTGCCAAACACC | ||
| GAPDH: glyceraldehyde-3-phosphate dehydrogenase | Forward | AGCCACATCGCTCAGACAC | 60 |
| Reverse | GCCCAATACGACCAAATCC | ||