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. 2022 Feb 10;32(2):128–137. doi: 10.1089/thy.2021.0461

FIG. 5.

FIG. 5.

Disorganized microtubules in postnatal and embryonic thyroid glands in Xb130−/− mice. (A, C, D) IF staining of acetylated tubulin in thyroid glands at different developmental stages. (A) At PW2, enhanced staining especially in the cytoplasm along the lateral membrane is seen in thyrocytes from Xb130−/− mice, with irregular follicle lumen structures (n = 6 mice in each group). (B) Protein expression of α-tubulin and acetylated tubulin in the thyroid gland of postnatal mice. Each lane represents a pool of three to four thyroid glands. (C) E15.5. Upper: low magnification. Lower: high magnification of the image in the white box. Increased staining is seen in thyrocytes from Xb130−/− mice (n = 8–11 mice in each group). (D) E18.5. Enhanced staining of elongated microtubule bundles in the cytoplasm, longitudinally parallel to or along the lateral membrane, is seen in thyrocytes from Xb130−/− mice (n = 4–6 mice in each group). (E) Proposed models of acetylated tubulin distribution in thyroid follicles from Xb130+/+ or from Xb130−/− mice. Color images are available online.