Fig. 8.

Specific deletion of Evc2 in neural crest–derived tissues leads to patent ISS at P2. Control (A), Evc2 mutant (D), and Evc2 Wnt1 mutant (G) braincase floor at P2 were sagittal sectioned and stained with H&E. The enlarged ISS and SOS for control (B, C), Evc2 mutant (E, F), and Evc2‐Wnt1‐Cre conditional mutants (H, I) are shown. Brackets indicate regions enlarged. Scale bar = 200 μm. Ptch1 (J), Ihh (K), and Evc2 (L) expression levels in controls and Evc2‐Wnt1‐Cre conditional mutant braincase floors were assayed through qRT‐PCR at E17.5. (n = 4, **p < 0.01, error bars denote standard deviations.)