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. 2022 Feb 4;13:800986. doi: 10.3389/fphar.2022.800986

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Copyright © 2022 Mahmoud, Nabil, Hasan, El-Shazly, El-Ansari and Sobeh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemical staining of inducible nitric oxide synthase (iNOS) in gastric mucosal tissues. (A) Control and (E) famotidine-pretreated ulcer groups furnished a minimal positive cytoplasmic immunoreactivity to iNOS in the gastric mucosal cells (arrows). (B) Ulcerative animals (indomethacin group) displayed an intense positive immune reaction to iNOS in the gastric mucosal cells (arrows). (C,D) PGG -pretreated ulcer groups (100 or 200 mg/kg) showed a reduction in iNOS immunoreactivity (arrows). iNOS immunostaining (avidine biotin peroxidase stained with Hx counter stain X 200, scale bar = 100 µm). (F) Quantitative analysis of immunoreactivity intensity of iNOS. Results are shown as mean ± SEM, n = 10. * ^{@, #}significantly different compared the control group, indomethacin, and famotidine groups, respectively, at p < 0.05 by one-way ANOVA followed by Tukey’s post hoc test.