Figure 3.

Safranal suppressed HUVECs invasion. Cells were seeded in the upper chamber of Transwell coated with matrigel and incubated with various concentrations of Safranal. The bottom chamber was added with culture medium with 30ng/ml rhVEGF. 24 hrs later, the nucleus of the migrated or invaded cells were stained with crystal violet (A) or DAPI (B). The cells were quantified (C) through manual counting and presented as the mean ± standard deviation of three independent experiments performed in triplicate. Statistical analysis was carried out by student’s t-test using GraphPad Prism software and p < 0.05 was considered as statistically significant. *p < 0.05.