Table 2.

Viral vectors for delivery of CRISPR/Cas9 system

Delivery vehicle	Packaging capacity	Advantages	Disadvantages
Adenovirus	Approximately 8-10 kb	Efficient delivery Large cargo size	Inflammatory response
Adeno-associated virus (AAV)	Approximately 4.7 kb	Multiple serotypes Low immunogenicity Can transduce dividing and non-dividing cells in different tissues	Pre-existing neutralizing antibodies Long-term expression of Cas9 causing off-target effects
Lentivirus	Approximately 10 kb	High transduction efficiency Large cargo size Low immunogenicity Can transduce dividing and non-dividing cells in different tissues	Non-specific DNA integration causing cancer risk Complex packaging structure