FIG. 1.

RFLP analysis of H. pylori vacA gene. A 1,161-bp region was amplified by PCR and digested with HaeIII, and the fragments were separated in a 2% agarose gel. (a) Homogeneous pattern characterized by three bands. Lanes A and R, molecular size marker VI; lanes B to O, isolates from 14 patients with ulcer dyspepsia, respectively; lanes P and Q, isolates from patients with nonulcer dyspepsia. (b) Heterogeneous profiles characterized by several different patterns. Lanes A and R, molecular size marker VI; lanes B to I, isolates from asymptomatic H. pylori-infected patients; lanes J to O, isolates from patients with nonulcer dyspepsia; lane P, running buffer alone; lane Q, isolate from a patient with ulcer dyspepsia.