Fig. 4.

Altered pancreatic adipocyte area in mice with reduced hyperinsulinemia. A–B Representative high-magnification image of apparent adipocyte replacement of pancreas area in male (A) and female (B) PK-Ins1^-/-;Ins2^+/+ (left) and PK-Ins1^-/-;Ins2^+/- (right) mice stained with H&E. Black arrows point to residual ducts. Arrowheads point to remaining islets, acinar cells, and blood vessel. Scale bars: 0.5 mm. C–D Quantification of percent of total pancreatic area occupied by adipocytes in male and female mice of each genotype (n= 11–22). Correlations of adipocyte area with fasting insulin levels (E–F), fasting glucose levels (G–H), or body weight (I–J) for male (E, G, I) and female (F, H, J) PK-Ins1^-/-;Ins2^+/+ (dark colors) and PK-Ins1^-/-;Ins2^+/- (light colors) mice (n = 11–22). Values are shown as mean ± SEM