FIGURE 1.

Improving the solubility of lanFP10A. (a) Amino acid sequence of proteins generated during improvement of solubility of lanFP10A; the chromophore‐forming triad is highlighted in yellow. bfVFP was added to the sequence alignment. (b) Phenotype of E. coli cells expressing the proteins whose sequence is shown above. (c) SDS–PAGE of cells expressing the proteins shown above revealed by Western blot using anti‐His antibody coupled to alkaline phosphatase. M: protein marker, 1 and 2: lanFP10A, 3 and 4 lanFP10A2, 5 and 6: lanFP10A3, 7 and 8: lanFP10A2‐F70Y. Samples 1, 3, 5, and 7: soluble fractions; samples 2, 4, 6, and 8: total extracts