Figure 3.

Regulation of frequency in immune subsets associated with humoral immune response. The frequencies of (A) Memory B cells, (B) Naïve B cells, (C) Memory:Naïve B cell ratio, (D) Plasmablasts and (E) Circulating PD1⁺ cTfh. The frequencies in (A), (B), and (D) are shown as percentage of CD19⁺ and (E) as percentage of CD4⁺. Representative flow cytometry plots for subsets at each timepoint are shown in (F–H). (F) Zebra plots showing naïve and memory B cell subsets, with numbers on the plots indicating their percentages gated from CD19⁺ parent population. (G) Zebra plots showing plasmablast (PB) subset, with numbers on the plots indicating PB percentage gated from CD19⁺ parent population. (H) Zebra plots showing PD1⁺ cTfh subset, with numbers on the plots indicating PD1⁺ cTfh percentages gated from CD45RA⁻ parent population. Gating strategy can be obtained in Figure S2. Statistical analysis was performed using linear mixed‐effects model (p < 0.05) and multiple comparisons adjusted using Holm‐Sidak method. Logarithmic transformations were performed for analysing difference between pre‐AHSCT and post‐AHSCT timepoint in Memory B cells, Memory:Naïve B cell ratio and Plasmablasts. Statistical analysis between MS at pre‐/36M post‐AHSCT and HCs was performed using independent two‐sample t‐tests (p < 0.05). MS Pre‐AHSCT (Pre‐Tx) n = 20, 24 months (24M) n = 22, 36 months (36M) n = 22, HCs n = 18. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Grey bars and asterisks indicate cross‐sectional comparison between MS at pre‐/36M post‐AHSCT and HC cohorts, whereas black bars and asterisks indicate longitudinal comparison between pre‐ and post‐AHSCT timepoints within MS cohort.