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. 2022 Feb 22;22:96. doi: 10.1186/s12935-022-02513-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — CAFs-MVs-derived miR-223-3p can facilitate malignant progression of GC. A PKH26-labeled MVs co-incubated with GC cells (PKH26 in red while DAPI in blue) (n = 3, p < 0.0001; p < 0.001; scale bar: 20 μm); B miR-223-3p level in the CAFs (at logarithmic phase, n = 3, p = 0.0237) and MVs of CAFs (n = 3, p = 0.0021) in each transfection group (inhibitor NC, inhibitor); C miR-223-3p level in GC cells after 24 h of MVs co-cultured with GC cells (n = 3; p < 0.001; p < 0.001, n = 3; p = 0.0003; p = 0.0006); D The activity of GC cells after co-culture with MVs in each transfected group (control, inhibitor NC, inhibitor) (n = 3; p < 0.001; p = 0.0028, n = 3; p = 0.0011; p = 0.0214); E The colony-forming ability of GC cells after co-culture with MVs in each transfection group (n = 3; p = 0.0125; p = 0.0459, n = 3; p = 0.0062; p = 0.0023); F The migration and invasion of GC cells after co-culture with MVs in each transfected group (BGC-823 cells: n = 3; p < 0.001; p < 0.001, n = 3; p = 0.0001; p = 0.0003, AGS cells: n = 3; p = 0.0001; p < 0.0001, n = 3; p = 0.0015; p = 0.0007); G Cell apoptosis status (n = 3; p < 0.0001; p = 0.0002, n = 3; p = 0.0003; p = 0.0002); H Cell cycle status (n = 3; p = 0.0106; p = 0.0350, n = 3; p = 0.0101; p = 0.0224); I The expression of EMT-related proteins in GC cells after co-culture with MVs 24 h in each transfected group (BGC-823 cells: n = 3; p < 0.0001; p < 0.0001, p < 0.0001; p = 0.0006, p = 0030; p = 0007, AGS cells: p < 0.0001; p < 0.0001, p = 0.0003; p = 0.0002, p < 0.0001; p < 0.0001). *p < 0.05; **p < 0.01; ***p < 0.001

Fig. 3 — CAFs-MVs-derived miR-223-3p can facilitate malignant progression of GC. A PKH26-labeled MVs co-incubated with GC cells (PKH26 in red while DAPI in blue) (n = 3, p < 0.0001; p < 0.001; scale bar: 20 μm); B miR-223-3p level in the CAFs (at logarithmic phase, n = 3, p = 0.0237) and MVs of CAFs (n = 3, p = 0.0021) in each transfection group (inhibitor NC, inhibitor); C miR-223-3p level in GC cells after 24 h of MVs co-cultured with GC cells (n = 3; p < 0.001; p < 0.001, n = 3; p = 0.0003; p = 0.0006); D The activity of GC cells after co-culture with MVs in each transfected group (control, inhibitor NC, inhibitor) (n = 3; p < 0.001; p = 0.0028, n = 3; p = 0.0011; p = 0.0214); E The colony-forming ability of GC cells after co-culture with MVs in each transfection group (n = 3; p = 0.0125; p = 0.0459, n = 3; p = 0.0062; p = 0.0023); F The migration and invasion of GC cells after co-culture with MVs in each transfected group (BGC-823 cells: n = 3; p < 0.001; p < 0.001, n = 3; p = 0.0001; p = 0.0003, AGS cells: n = 3; p = 0.0001; p < 0.0001, n = 3; p = 0.0015; p = 0.0007); G Cell apoptosis status (n = 3; p < 0.0001; p = 0.0002, n = 3; p = 0.0003; p = 0.0002); H Cell cycle status (n = 3; p = 0.0106; p = 0.0350, n = 3; p = 0.0101; p = 0.0224); I The expression of EMT-related proteins in GC cells after co-culture with MVs 24 h in each transfected group (BGC-823 cells: n = 3; p < 0.0001; p < 0.0001, p < 0.0001; p = 0.0006, p = 0030; p = 0007, AGS cells: p < 0.0001; p < 0.0001, p = 0.0003; p = 0.0002, p < 0.0001; p < 0.0001). *p < 0.05; **p < 0.01; ***p < 0.001