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. Author manuscript; available in PMC: 2022 Feb 22.
Published in final edited form as: J Biomed Mater Res A. 2019 Jan 21;107(4):704–718. doi: 10.1002/jbm.a.36603

FIGURE 6.

FIGURE 6.

GFAP expression by NS/PCs increases with 2D culture and differentiation conditions. (A) GFAP mRNA was evaluated with RT-PCR in 2D and 3D hydrogels. In 2D, both PREDIFF (day 42) and full differentiation (PREDIFF, days 28–42 + DIFF, days 43–70) conditions significantly increased GFAP expression compared to 2D STEMRPO (day 28) (#). Both PREDIFF (day 42) and differentiation (day 70) conditions in 3D cultures showed a statistically significant decrease in GFAP expression compared to NS/PCs cultured in the same media conditions in 2D (*). Error bars show the standard deviation of multiple experiments. Error bars show the standard deviation (N ≥ 4). (B) NS/PCs cultured in either 2D (top) or 3D with YIGSR, IKVAV, and RGD peptides (bottom) were immunofluorescently labeled for GFAP (red), a marker of activated astrocytes. Images are 200×; inset images are 1000×. Media conditions: stem cell maintenance (STEM), oligodendrocyte-lineage conditioning (PRE-DIFF), and oligodendrocyte differentiation (DIFF).