Fig. 5.

circCUL2 is a sponge of miR-203a-3p. A Subcellular localization of circCUL2 in CAFs detected by FISH. Scale bar, 50 μm. B circCUL2 expression in cytoplasm and nucleus of CAFs was detected by qRT–PCR. GAPDH and U6 RNA were used as cytoplasmic and nuclear RNA markers, respectively. C Schematic illustration of the potential target miRNAs of circCUL2 predicted by CircInteractome. D-E qRT–PCR analysis of the indicated miRNAs enriched with biotin-labeled circCUL2 probes in NFs and CAFs. F Diagram of the secondary structure of circCUL2 and the possible binding sites with miR-203a-3p predicted by RNAalifold. G-H Luciferase reporter assay was used to detect the luciferase activity of circCUL2-WT and circCUL2-mut (miR-203a-3p binding site mutated) luciferase reporter cotransfected with miR-203a-3p mimic. N.S., no significant. I qRT–PCR analysis of circCUL2 enriched with biotin-labeled miR-203a-3p probes in NFs and CAFs. J Subcellular localization of circCUL2 and miR-203a-3p in CAFs detected by FISH. Scale bar, 50 μm. Data are expressed as the mean ± SD. ^**p < 0.01 and ^***p < 0.001