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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Mol Microbiol. 2021 Apr 16;116(2):427–437. doi: 10.1111/mmi.14721

FIGURE 2.

FIGURE 2

The bacterial biosensor with the antR gene responds only to Sb(III) and MAs(III). Conditions for constitutive, repressed, or derepressed gfp expression. In cells of E. coli AW3110 with both plasmids, antR is not expressed in the absence of arabinose, and gfp expression is constitutive, producing cellular fluorescence. In the presence of arabinose, antR is expressed, and gfp is repressed, so the cells are not fluorescent. In the presence of both arabinose and arsenical inducer, gfp expression is derepressed, and the cells are fluorescent. Expression of the gfp reporter gene was assayed as described under Experimental Procedures. Cells of E. coli strain AW3110(DE3) bearing wild-type antR were grown without arabinose, 0.2% arabinose, or 0.2% arabinose and metalloids at the indicated concentrations. Fluorescence intensities were quantified by spectrofluorometry. The data are the mean ± SE (n = 3)