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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Mol Microbiol. 2021 Apr 16;116(2):427–437. doi: 10.1111/mmi.14721

FIGURE 3.

FIGURE 3

Role of cysteine residues in binding of Sb(III) and MAs(III) to AntR. Expression of the gfp reporter gene was assayed as described under Experimental Procedures. Cells were grown in low phosphate medium for 14 hr with 0.5% glycerol as carbon source. Cells of E. coli strain AW3110(DE3) bearing plasmids with wild-type AntR, C103S, or the C113S mutant in trans with reporter plasmid pACYC184-ParsP-gfp were grown with 0.2% arabinose and the indicated concentrations of metalloids. (a), Comparison of the response of the bacterial biosensor to arsenicals and antimonite. Response mutant biosensors (b) C58S; (c) C103S; and (d) C113S to the indicated inducers indicated. Fluorescence intensities of cell suspensions were quantified using a Photon Technology International Spectrofluorometer with an excitation wavelength of 470 nm and emission wavelength of 510 nm. The data are the mean ± SE (n = 3)