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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Mol Microbiol. 2021 Apr 16;116(2):427–437. doi: 10.1111/mmi.14721

FIGURE 6.

FIGURE 6

AntR negatively regulates the expression of the ant promoter. (a) The FAM-labeled antR promoter probe interacts with purified AntR, retarding the electrophoretic mobility. The amounts of DNA probes and AntR are shown above each panel. Binding of substrate releases AntR from the DNA, increasing the electrophoretic mobility. Metal(loids) were (b) Sb(III); (c) As(III); (d), As(V); (e) Cu(II); or (f), Zn(II) at the indicated concentrations. The amount of DNA probe was 100 ng, the amount of AntR was 1.0 μM