Skip to main content
. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: Curr Protoc. 2021 May;1(5):e129. doi: 10.1002/cpz1.129

Figure 4. Differentiation of N-linked and O-linked glycans.

Figure 4.

Lysates from cells exposed to control conditions or nutrient deprivation (STV: 0mM glucose, 1% dialyzed FBS) were treated with ±PNGase F (PF), prior to separation by SDS-PAGE and transferred to PVDF. Membranes were incubated in A) water or B) 55mM NaOH overnight at 40°C. After blocking, membranes were probed for O-GlcNAc (CTD110.6) or Actin. Blots were developed using ECL and images captured on film. These data are adapted from Reeves et al., 2014 (Reeves et al., 2014).