Table 1.

Antibodies and Lectins that Recognize O-GlcNAc and GlcNAc

Name	Antibody isotype	Recognizes	Commercially available	Positive control	Negative control	Reference
CTD110.6	IgM	βGlcNAc	Covance, Pierce, Millipore SIGMA, Cell Signaling, Santa Cruz Biotechnology	BSA-AP-GlcNAc	BSA-APa or ovalbumin	(Comer et al., 2001)
RL2	IgG	O-GlcNAc	Abcam, Affinity Bioreagents, Santa Cruz Biotechnology	BSA-AP-GlcNAc	BSA-AP or ovalbumin	(Snow et al., 1987)
MY95	IgG	O-GlcNAc	No	BSA-AP-GlcNAc	BSA-AP or ovalbumin	(Matsuoka et al., 2002)
18B10.C7(3)	IgG	O-GlcNAc	Millipore	BSA-AP-GlcNAc	BSA-AP or ovalbumin	(Teo et al., 2010)
9D1.E4(10)	IgG	βGlcNAc	Millipore	BSA-AP-GlcNAc	BSA-AP or ovalbumin	(Teo et al., 2010)
1F5.D6(14)	IgG	O-GlcNAc	Millipore	BSA-AP-GlcNAc	BSA-AP or ovalbumin	(Teo et al., 2010)
HGAC 85	IgG	βGlcNAc	Novus Biologicals, Abcam, Pierce, Enzo life sciences, Affinity Bioreagents	BSA-AP-GlcNAc	BSA-AP or BSA	(Turner et al., 1990)
HGAC 49	IgM	βGlcNAc	No	BSA-AP-GlcNAc	BSA-AP or BSA	(Turner et al., 1990)
HGAC 39	IgG	βGlcNAc	No	BSA-AP-GlcNAc	BSA-AP or BSA	(Turner et al., 1990)
Multi-mAbb	IgG	O-GlcNAc	Cell Signaling Technology		BSA	N/A

^aBSA-AP, BSA-aminophenol.

^bThis antibody was raised against glycoproteins. Modulating O-GlcNAc levels is an appropriate control.