Table 3.
Sample | Dilution | Vol. (μl) of diluted sample | Vol. (μl) of 200 mM GlcNAc | Vol. (μl) 10× Gal labeling buffer | Vol. (μl) Milli-Q water |
---|---|---|---|---|---|
Blank | 0 | 10 | 10 | 70 | |
Pre-Gal | 1/1000 | 10 | 10 | 10 | 60 |
1/10,000 | 10 | 10 | 10 | 60 | |
1/100,000 | 10 | 10 | 10 | 60 | |
Auto-Gal | 1/1000 | 10 | 10 | 10 | 60 |
1/10,000 | 10 | 10 | 10 | 60 | |
1/100,000 | 10 | 10 | 10 | 60 |