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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: Curr Protoc. 2021 May;1(5):e129. doi: 10.1002/cpz1.129

Table 3.

Reaction Mixtures for Assay of Galactosyltransferase Activity

Sample Dilution Vol. (μl) of diluted sample Vol. (μl) of 200 mM GlcNAc Vol. (μl) 10× Gal labeling buffer Vol. (μl) Milli-Q water
Blank 0 10 10 70
Pre-Gal 1/1000 10 10 10 60
1/10,000 10 10 10 60
1/100,000 10 10 10 60
Auto-Gal 1/1000 10 10 10 60
1/10,000 10 10 10 60
1/100,000 10 10 10 60