TABLE 3.
Evaluation of three serologic assays for the detection of H5-specific antibody in confirmed and suspected H5N1 virus-infected individuals and nonexposed controls
| Age group | Serum | Sample | H5-specific antibody response
|
|||
|---|---|---|---|---|---|---|
| Na | ELISAb
|
WBa | ||||
| IgG | IgM | |||||
| Children | ||||||
| B | S1 | 10 | 5.6 | 5.6 | − | |
| S2 | 80 | 16.6 | 5.6 | +d | ||
| F | S2 | 14 | 14.6 | 16.6 | +d | |
| G | S1 | 10 | 5.6 | 8.6 | − | |
| S2 | 320 | 14.6 | 14.6 | +d | ||
| H | S1 | 10 | 5.6 | 5.6 | NTe | |
| S2 | 450 | 18.6 | 16.6 | + | ||
| Control (n = 24) | 11 | 6.0 | 5.6 | |||
| Adults | ||||||
| A | S1 | 20 | 18.6 | 5.6 | + | |
| S2 | 160 | 18.6 | 5.6 | + | ||
| D | S1 | 10 | 6.6 | 6.6 | − | |
| S2 | 320 | 18.6 | 18.6 | +d | ||
| I | S1 | 10 | 5.6 | 5.6 | − | |
| S2 | 1,280 | 18.6 | 18.6 | + | ||
| J | S2 | 10 | 5.6 | 5.6 | − | |
| Control (n = 46) | 20 | 11.0 | 6.0 | |||
N, microneutralization assay; GMT to A/Hong Kong/156/97 virus.
GMT (log2).
W, Western blotting. +, positive; −, negative.
Serum also positive with IgM.
NT, not tested.