TABLE 1.
Reference isolates used to determine specificities of five automated microbial identification systems for common food-borne pathogens
| Pathogen | Reference positive isolates | Reference negative isolates |
|---|---|---|
| B. cereus | 35 laboratory isolates, ATCC 4342, ATCC 14579, ATCC 7004, ATCC 33018, ATCC 11778 | 16 Bacillus spp. (non-B. cereus), 2 Bacillus amyloliquifaciens, 1 Bacillus circulans, 1 Bacillus megaterium, 1 Bacillus sphaericus, 3 Bacillus subtilis, 3 Bacillus lichenformis, 2 Bacillus pumulis, 1 Bacillus pasteurii, 3 Bacillus circulans, 1 Bacillus lentus, 2 E. coli, 1 S. aureus, 1 Staphylococcus haemolyticus, 1 L. monocytogenes, 1 Listeria seeligeri |
| C. jejuni | 35 laboratory isolates, ATCC 49349, ATCC 33560, ATCC 29428, ATCC 43432, ATCC 33291 | 29 C. coli, 3 Campylobacter lari, 1 Campylobacter fetus, 1 Campylobacter hyointenstinalis, 5 Ochrobactum anthropi, 1 Pseudomonas aeruginosa |
| L. monocytogenes | 35 laboratory isolates, ATCC 19111, ATCC 19112, ATCC 19117, ATCC 19114, ATCC 19116 | 15 Listeria innocua, 10 Listeria welshimeri, 7 Listeria seeligeri, 2 Listeria murrayi, 1 Listeria ivanovii, 1 Listeria grayi, 1 S. haemolyticus, 1 Staphylococcus epidermidis, 1 Streptococcus agalactiae, 1 R. equi |
| S. aureus | 35 laboratory isolates, ATCC 25923, ATCC 29213, ATCC 9144, ATCC 12600, ATCC 27154 | 7 Staphylococcus intermedius, 6 Staphylococcus xylosus, 5 Staphylococcus cohnii subsp., 4 Staphylococcus schleiferi, subsp., 4 S. haemolyticus, 2 Staphylococcus lugdunensis, 4 Staphylococcus hyicus, 1 Staphylococcus sciuri, 2 Staphylococcus saprophyticus, 3 Staphylococcus epidermidis, 1 Staphylococcus chromogenes, 1 Micrococcus luteus |
| Verotoxigenic E. coli | 35 laboratory isolates, ATCC 43887, ATCC 43889, ATCC 43895, ATCC 43894, ATCC 25922 | 14 Enterobacter spp., 6 Klebsiella spp., 8 Citrobacter spp., 2 Hafnia alvei, 2 Serratia spp., 1 Yersinia enterocolitica, 1 Pseudomonas alcaligenes, 1 Acinetobacter baumanii, 1 P. aeruginosa, 1 Kluyvera ascorbata, 3 Shigella spp. |
| Salmonella spp. | 35 laboratory isolates, ATCC 8326, ATCC 8391, ATCC 6962, ATCC 13076, ATCC 14028 | 14 Enterobacter spp., 6 Klebsiella spp., 2 H. alvei, 2 Serratia spp. 1 Y. enterocolitica, 10 Citrobacter spp., 3 E. coli, 1 Edwardsiella tarda, 1 S. sonnei |