Extended Data Figure 5. Genetic stability analysis of Δ3678 SARS-CoV-2.

a, Experimental scheme. Passage 0 (P0) Δ3678 virus was divided into three T25 flasks for five rounds of independent passaging on Vero-E6 cells. b, Plaque morphologies of P0 and P5 Δ3678 virus. c, Mutations recovered from three independently cultured P5 Δ3678 viruses. The P5 Δ3678 viral RNAs were extracted and amplified by RT-PCR. Whole-genome sequencing was performed on the RT-PCR products. Mutations from the P5 viruses were annotated with amino acid changes and specific genes.