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. Author manuscript; available in PMC: 2022 Feb 28.
Published in final edited form as: Oncogene. 2021 Aug 31;40(42):6071–6080. doi: 10.1038/s41388-021-01994-0

Figure 7. PHF10 cooperates with MYC dependent transcription in A375 melanoma cells.

Figure 7.

(A) Presence of PHF10 and MYC on the regulatory regions of MYC-dependent genes (ChIP analysis). Regions included the promoters and proximal (+/−150 bp) MYC binding cites. (B) Left: levels of PHF10 and MYC mRNAs in control (siCont) and PHF10 (siPHF10) knockdown cells after serum addition (+FBS). MYC mRNA level in serum starved cells (-FBS) was taken as 1. Middle: serum replenishment does not change PHF10 mRNA abundance after PHF10 down-regulation. PHF10 mRNA level in cells treated with siControl and serum starved was taken as 1. Right: serum replenishment activates MYC responsive genes in a PHF10 dependent manner Shown are the ratios of transcription of an individual gene after serum replenishment to the respective value in serum starved, siControl treated (grey bars) or PHF10 knockdown (black bars) cells (mean ± SD from three independent experiments). Statistically significant differences were marked as *(p < 0.05 for pairs of compared data (one-way ANOVA, Holm-Sidak's multiple comparison test).