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. 2022 Feb 22;17(2):e0263194. doi: 10.1371/journal.pone.0263194

Table 2. Mean square values for biochemical and yield attributes of spinach (Spinaceae oleraceae L.) plants with priming and foliar application of ZnNPs under saline conditions.

Source of variation Salinity (S) Treatment (T) ZnNPs Salinity * Treatment Error
Shoot Length 475.373*** 24.506ns 5.246ns 13.3
Root Length 50.82* 8.088ns 2.983ns 7.381
Shoot fresh weight 1669.627*** 114454ns 39.496ns 54.221
Root fresh weight 32.138*** 4.494* 1.441ns 1.324
Shoot dry weight 17.012*** 1.889* 1.189ns 0.701
Root dry weight 1.452*** 0.232** 0.097ns 0.057
No. of leaves 64.381** 20.873* 5.381ns 6.929
Chl a 2.827*** 0.069ns 0.057ns 0.035
Chl b 0039*** 0.001ns 0.002ns 0.003
Chl t 3.529*** 0.060ns 0.047ns 0.035
Carotenoids 3.975** 8.643ns 3.235ns 3.690
No. of leaves 64.381** 20.873* 5.381ns 6.929
Anthocyanin 42.681*** 0.610ns 1.588ns 2.406
Total soluble proteins 6.512*** 0.0583ns 0.042ns 0.083
Total soluble sugars 0.0079*** 4.1522ns 6.675ns 4.796
Total free amino acids 0.345** 0.018ns 0.004ns 0.039
Phenolics 0.645ns 0.925ns 0.936ns 0.503
Flavonoid contents 0.014 0.013ns 0.022ns 0.009
Reducing sugars 47278.999ns 47099.906ns 47320.075ns 4.723
MDA 116.022*** 1.077ns 0.479ns 9.643
CAT 10188.269*** 82.879ns 813.617ns 517.357
POD 3446.346ns 38.686ns 182.530ns 961.884
SOD 2043.732ns 1392.351ns 2105.156ns 1004.179
H2O2 0.0013ns 0.0039* 0.0012ns 0.001
Ascorbic acid 14.081*** 1.507* 0.865 0.506
df 1 6 6 28

Df degrees of freedom, ns nonsignificant, ***, **Significant at 0.001, 0.01 and *at 0.05 level of significance at 5% probability.