Integration of metabolic technologies with force spectroscopy modalities correlate metabolic profiles to mechanics at different scales (blue bars) and in variable conditions. Future advances may improve the application of these techniques to single cells.
Met-Flow, a high-parameter flow cytometry method utilizing antibodies against metabolic proteins; scMEP, single-cell metabolic regulome profiling; MIBI-TOF, multiplexed ion beam imaging by time of flight; MALDI, matrix-assisted laser desorption/ionization; DESI, desorption electrospray ionization; SIMS, secondary ion mass spectrometry. Mass spectrometer and flow cytometry illustrations were generated using BioRender.