Table 2. Summary of published conditions to culture Drosophila imaginal discs ex vivo. Adapted from Tsao et al., 2016.

Reference*	Medium	Serum (v/v)	Antibiotic	Fly extract (v/v)	Insulin	Ecdysone	Sample type	Duration
Robb, 1969	R-14	---	1X	---	---	---	wing disc	---
Davis and Shearn, 1977	X (XCS)	---	---	---	0.4 mU/ml	1 ng/ml	discs	---
Wyss, 1982	ZW	??? FBS	---	22.5%	10 mg/ml	10 ng/ml	disc cells	---
Currie et al., 1988	Shields and Sang M3	2% FBS	---	5%	125 mU/ml	1 ng/ml	disc cells	---
Schubiger and Truman, 2000	Shields and Sang M3 D22	7.5% FCS	---	---	---	1 mg/ml	wing disc	24 hr
Gibson et al., 2006	Shields and Sang M3	10% FBS	1X	---	0.01 mU/ml	---		1.5–2 hr
Cafferty et al., 2009	Schneider’s	1% FBS	10 X	---	200 mg/ml	---		4 hr
Landsberg et al., 2009	Shields and Sang M3	2% FCS	1X	2.5%	0.125 iu/ml	---	wing disc cells
Aldaz et al., 2010	Shields and Sang M3	2% FCS	5X	---	---	100–500 ng/ml	disc eversion	10 hr
Mao et al., 2011	Shields and Sang M3	2% FBS	1X	---	0.01 mU/ml	---	wing disc	5 hr
Ohsawa et al., 2012	Schneider’s	10% FBS	---	---	---	---		3 hr
Zartman et al., 2013	Schneider’s	variable	4X	5%	6.2 µg/ml	---	wing disc	5 hr
Legoff et al., 2013	Shields and Sang M3	2% FCS	---	2.5%	125 mU/ml	---	wing disc	8 hr
Handke et al., 2014	Shields and Sang M3	2% FBS	10 X	5%	5 µg/ml	1 ng/ml	wing disc	7 hr
Tsao et al., 2016	Schneider’s	2% FBS	4X	---	1250 µg/ml	---	multiple discs	18 hr
Dye et al., 2017	Grace’s Insect Medium	5% FBS	1X	---	---	1 ng/ml	wing discs	12+ hr
This study	Grace’s Insect Medium	2% FBS	1X	---	625 µg/ml	---	eye disc	12–16 hr