| Genetic reagent(Escherichia coli) |
DH5α |
Hanahan and Glover, 1985
|
F–
endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZ∆M15 ∆(lacZYA-argF)U169 hsdR17(rK–mK+) λ–
|
- |
| Genetic reagent(Escherichia coli) |
CC118λpir |
Herrero et al., 1990
|
araD Δ(ara, leu) ΔlacZ74 phoA20 galK thi-1 rspE rpoB argE recA1 λpir
|
- |
| Genetic reagent(Escherichia coli) |
HB101 |
Boyer and Roulland-Dussoix, 1969
|
supE44 hsdS20 recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 |
- |
| Genetic reagent(Escherichia coli) |
MC1000 |
Casadaban and Cohen, 1980
|
araD139 ∆(ara, leu)7697 ∆lacX74 galU galK strA |
- |
| Genetic reagent(Escherichia coli) |
MC1000 dsbA
|
Kadokura et al., 2004
|
dsbA::aphA, KanR
|
- |
| Genetic reagent(Escherichia coli) |
MC1000 dsbA attTn7::Ptac-dsbA
|
This study |
dsbA::aphA attTn7::dsbA, KanR
|
Can be obtained from the Mavridou lab |
| Genetic reagent(Escherichia coli) |
MG1655 |
Blattner et al., 1997
|
K-12 F– λ– ilvG–
rfb-50 rph-1 |
- |
| Genetic reagent(Escherichia coli) |
MG1655 dsbA
|
This study |
dsbA::aphA, KanR
|
Can be obtained from the Mavridou lab |
| Genetic reagent(Escherichia coli) |
MG1655 dsbA attTn7::Ptac-dsbA
|
This study |
dsbA::aphA attTn7::dsbA, KanR
|
Can be obtained from the Mavridou lab |
| Genetic reagent(Escherichia coli) |
MG1655 acrA
|
This study |
acrA
|
Can be obtained from the Mavridou lab |
| Genetic reagent(Escherichia coli) |
MG1655 tolC
|
This study |
tolC
|
Can be obtained from the Mavridou lab |
| Genetic reagent(Escherichia coli) |
MG1655 degP
|
This study |
degP::strAB, StrR
|
Can be obtained from the Mavridou lab |
| Strain, strain background (Escherichia coli) |
BM16 |
Dortet et al., 2014
|
bla
TEM-1b
bla
KPC-2
|
Human clinical strain |
| Strain, strain background (Escherichia coli) |
LIL-1 |
Dortet et al., 2014
|
blaTEM-1blaOXA-9
blaKPC-2
|
Human clinical strain |
| Strain, strain background (Escherichia coli) |
CNR1790 |
Dortet et al., 2018
|
blaTEM-15
mcr-1
|
Human clinical strain |
| Strain, strain background (Escherichia coli) |
CNR20140385 |
Dortet et al., 2018
|
bla
OXA-48
mcr-1
|
Human clinical strain |
| Strain, strain background (Escherichia coli) |
WI2 (ST1288) |
Beyrouthy et al., 2017
|
blaOXA-48blaKPC-28
mcr-1
|
Human clinical strain |
| Strain, strain background (Escherichia coli) |
1073944 (ST117) |
Wise et al., 2018
|
mcr-1
|
Human clinical strain |
| Strain, strain background (Escherichia coli) |
41,489 |
Dortet et al., 2018
|
mcr-1
|
Human clinical strain |
| Strain, strain background (Escherichia coli) |
- |
Dortet et al., 2018
|
mcr-1
|
Human clinical strain |
| Strain, strain background (Escherichia coli) |
1256822 (ST48) |
Wise et al., 2018
|
mcr-1.5
|
Human clinical strain |
| Strain, strain background (Escherichia coli) |
27,841 (ST744) |
Haenni et al., 2018
|
bla
CTX-M-55
mcr-3.2
|
Environmental strain from livestock |
| Strain, strain background (Escherichia coli) |
1144230 (ST641) |
Wise et al., 2018
|
bla
CMY-2
mcr-5
|
Human clinical strain |
| Strain, strain background (Klebsiella pneumoniae) |
ST234 |
Nordmann et al., 2012
|
bla
SHV-27
bla
KPC-2
|
Human clinical strain |
| Strain, strain background (Citrobacter freundii) |
BM19 |
Dortet et al., 2014
|
bla
KPC-2
|
Human clinical strain |
| Strain, strain background (Enterobacter cloacae) |
DUB |
Dortet et al., 2015
|
bla
FRI-1
|
Human clinical strain |
| Strain, strain background (Pseudomonas aeruginosa) |
PA43417 |
El Garch et al., 2011
|
bla
OXA-198
|
Human clinical strain |
| Genetic reagent (Pseudomonas aeruginosa) |
PA43417 |
This study |
dsbA1 bla
OXA-198
|
Can be obtained from the Mavridou lab |
| Strain, strain background (Pseudomonas aeruginosa) |
PAe191 |
Mugnier et al., 1998
|
bla
OXA-19
|
Human clinical strain |
| Genetic reagent (Pseudomonas aeruginosa) |
PAe191 |
This study |
dsbA1 bla
OXA-19
|
Can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1 (plasmid) |
Lab stock |
GenBank MN128719
|
pDM1 vector, p15A ori, Ptac promoter, MCS, TetR
|
| Recombinant DNA reagent |
pDM1-blaL2-1 (plasmid) |
This study |
- |
blaL2-1 cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-blaGES-1 (plasmid) |
This study |
- |
blaGES-1 cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-blaGES-2 (plasmid) |
This study |
- |
blaGES-2 cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-blaGES-11 (plasmid) |
This study |
- |
blaGES-11 cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-blaSHV-27 (plasmid) |
This study |
- |
blaSHV-27 cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-blaOXA-4 (plasmid) |
This study |
- |
blaOXA-4 cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-blaOXA-10 (plasmid) |
This study |
- |
blaOXA-10 cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-blaOXA-198 (plasmid) |
This study |
- |
blaOXA-198 cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-blaFRI-1 (plasmid) |
This study |
- |
blaFRI-1 cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-blaL1-1 (plasmid) |
This study |
- |
blaL1-1 cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-blaKPC-2 (plasmid) |
This study |
- |
blaKPC-2 cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-blaKPC-3 (plasmid) |
This study |
- |
blaKPC-3 cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-blaSME-1 (plasmid) |
This study |
- |
blaSME-1 cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-mcr-1 (plasmid) |
This study |
- |
mcr-1 cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-mcr-3 (plasmid) |
This study |
- |
mcr-3 cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-mcr-4 (plasmid) |
This study |
- |
mcr-4 cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-mcr-5 (plasmid) |
This study |
- |
mcr-5 cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-mcr-8 (plasmid) |
This study |
- |
mcr-8 cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-blaL2-1-StrepII (plasmid) |
This study |
- |
blaL2-1 encoding L2-1 with a C-terminal StrepII tag cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-blaGES-1-StrepII (plasmid) |
This study |
- |
blaGES-1 encoding GES-1 with a C-terminal StrepII tag cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-StrepII-blaOXA-4 (plasmid) |
This study |
- |
blaOXA-4 encoding OXA-4 with an N-terminal StrepII tag cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-blaOXA-10-StrepII (plasmid) |
This study |
- |
blaOXA-10 encoding OXA-10 with a C-terminal StrepII tag cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-blaOXA-198-StrepII (plasmid) |
This study |
- |
blaOXA-198 encoding OXA-198 with a C-terminal StrepII tag cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-blaFRI-1-StrepII (plasmid) |
This study |
- |
blaFRI-1 encoding FRI-1 with a C-terminal StrepII tag cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-blaL1-1-StrepII (plasmid) |
This study |
- |
blaL1-1 encoding L1-1 with a C-terminal StrepII tag cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-blaKPC-3-StrepII (plasmid) |
This study |
- |
blaKPC-3 encoding KPC-3 with a C-terminal StrepII tag cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-mcr-1-StrepII (plasmid) |
This study |
- |
blaMCR-1 encoding MCR-1 with a C-terminal StrepII tag cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-mcr-3-StrepII (plasmid) |
This study |
- |
blaMCR-3 encoding MCR-3 with a C-terminal StrepII tag cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-mcr-4-StrepII (plasmid) |
This study |
- |
blaMCR-4 encoding MCR-4 with a C-terminal StrepII tag cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-mcr-5-StrepII (plasmid) |
This study |
- |
blaMCR-5 encoding MCR-5 with a C-terminal StrepII tag cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pDM1-mcr-8-StrepII (plasmid) |
This study |
- |
blaMCR-8 encoding MCR-8 with a C-terminal StrepII tag cloned into pDM1, TetR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pGRG25 (plasmid) |
McKenzie and Craig, 2006
|
- |
Encodes a Tn7 transposon and tnsABCD under the control of ParaB, thermosensitive pSC101 ori, AmpR
|
| Recombinant DNA reagent |
pGRG25-Ptac::dsbA (plasmid) |
This study |
- |
Ptac::dsbA fragment cloned within the Tn7 of pGRG25; when inserted into the chromosome and the plasmid cured, the strain expresses DsbA upon IPTG induction, AmpR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pSLTS (plasmid) |
Kim et al., 2014
|
- |
Thermosensitive pSC101ori, ParaB for λ-Red, PtetR for I-SceI, AmpR
|
| Recombinant DNA reagent |
pUltraGFP-GM (plasmid) |
Mavridou et al., 2016
|
- |
Constitutive sfGFP expression from a strong Biofab promoter, p15A ori, (template for the accC cassette), GentR
|
| Recombinant DNA reagent |
pKD4 (plasmid) |
Datsenko and Wanner, 2000
|
- |
Conditional oriRγ ori, (template for the aphA cassette), AmpR
|
| Recombinant DNA reagent |
pCB112 (plasmid) |
Paradis-Bleau et al., 2014
|
- |
Inducible lacZ expression under the control of the Plac promoter, pBR322 ori, CamR
|
| Recombinant DNA reagent |
pKNG101 (plasmid) |
Kaniga et al., 1991
|
- |
Gene replacement suicide vector, oriR6K, oriTRK2, sacB, (template for the strAB cassette), StrR
|
| Recombinant DNA reagent |
pKNG101-dsbA1 (plasmid) |
This study |
- |
PCR fragment containing the regions upstream and downstream P. aeruginosa dsbA1 cloned in pKNG101; when inserted into the chromosome the strain is a merodiploid for dsbA1 mutant, StrR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pRK600 (plasmid) |
Kessler et al., 1992
|
- |
Helper plasmid, ColE1 ori, mobRK2, traRK2, CamR
|
| Recombinant DNA reagent |
pMA-T mcr-3 (plasmid) |
This study |
- |
GeneArt cloning vector containing mcr-3, ColE1 ori, (template for mcr-3), AmpR; can be obtained from the Mavridou lab |
| Recombinant DNA reagent |
pMK-T mcr-8 (plasmid) |
This study |
- |
GeneArt cloning vector containing mcr-8, ColE1 ori, (template for mcr-8), KanR; can be obtained from the Mavridou lab |
| Chemical compound, drug |
Ampicillin |
Melford |
A40040-10.0 |
- |
| Chemical compound, drug |
Piperacillin |
Melford |
P55100-1.0 |
- |
| Chemical compound, drug |
Cefuroxime |
Melford |
C56300-1.0 |
- |
| Chemical compound, drug |
Ceftazidime |
Melford |
C59200-5.0 |
- |
| Chemical compound, drug |
Imipenem |
Cambridge Bioscience |
CAY16039-100 mg |
- |
| Chemical compound, drug |
Aztreonam |
Cambridge Bioscience |
CAY19784-100 mg |
- |
| Chemical compound, drug |
Kanamycin |
Gibco |
11815032 |
- |
| Chemical compound, drug |
Gentamicin |
VWR |
A1492.0025 |
- |
| Chemical compound, drug |
Streptomycin |
ACROS Organics |
AC612240500 |
- |
| Chemical compound, drug |
Tetracycline |
Duchefa Biochemie |
T0150.0025 |
- |
| Chemical compound, drug |
Colistin sulphate |
Sigma |
C4461-1G |
- |
| Chemical compound, drug |
Tazobactam |
Sigma |
T2820-10MG |
- |
| Chemical compound, drug |
Isopropyl β-D-1-thiogalactopyranoside (IPTG) |
Melford |
I56000-25.0 |
- |
| Chemical compound, drug |
KOD Hotstart DNA Polymerase |
Sigma |
71086–3 |
- |
| Chemical compound, drug |
Nitrocefin |
Abcam |
ab145625-25mg |
- |
| Chemical compound, drug |
1-N-phenylnaphthylamine (NPN) |
Acros Organics |
147160250 |
- |
| Chemical compound, drug |
4-acetamido-4ˊ-maleimidyl-stilbene-2,2ˊ-disulfonic acid (AMS) |
ThermoFisher Scientific |
A485 |
- |
| Chemical compound, drug |
4,5-dichloro-2-(2-chlorobenzyl)pyridazin-3-one |
Enamine |
EN300-173996 |
- |
| Commercial assay or kit |
BugBuster Mastermix |
Sigma |
71456–3 |
- |
| Commercial assay or kit |
Novex ECL HRP chemiluminescent substrate reagent kit |
ThermoFisher Scientific |
WP20005 |
- |
| Commercial assay or kit |
SigmaFast BCIP/NBT tablets |
Sigma |
B5655-25TAB |
- |
| Commercial assay or kit |
Immobilon Crescendo chemiluminescent reagent |
Sigma |
WBLUR0100 |
- |
| Commercial assay or kit |
ETEST - Amoxicillin |
Biomerieux |
412,242 |
- |
| Commercial assay or kit |
ETEST - Cefuroxime |
Biomerieux |
412,304 |
- |
| Commercial assay or kit |
ETEST - Ceftazidime |
Biomerieux |
412,292 |
- |
| Commercial assay or kit |
ETEST - Imipenem |
Biomerieux |
412,373 |
- |
| Commercial assay or kit |
ETEST - Aztreonam |
Biomerieux |
412,258 |
- |
| Commercial assay or kit |
ETEST - Gentamicin |
Biomerieux |
412,367 |
- |
| Commercial assay or kit |
ETEST - Erythromycin |
Biomerieux |
412,333 |
- |
| Commercial assay or kit |
ETEST - Chloramphenicol |
Biomerieux |
412,308 |
- |
| Commercial assay or kit |
ETEST - Nalidixic acid |
Biomerieux |
516,540 |
- |
| Commercial assay or kit |
ETEST - Ciprofloxacin |
Biomerieux |
412,310 |
- |
| Commercial assay or kit |
ETEST - Nitrofurantoin |
Biomerieux |
530,440 |
- |
| Commercial assay or kit |
ETEST - Trimethoprim |
Biomerieux |
412,482 |
- |
| Antibody |
Strep-Tactin-HRP conjugate (mouse monoclonal) |
Iba Lifesciences |
NC9523094 |
(1:3,000) in 3 w/v % BSA/TBS-T |
| Antibody |
Strep-Tactin-AP conjugate (mouse monoclonal) |
Iba Lifesciences |
NC0485490 |
(1:3,000) in 3 w/v % BSA/TBS-T |
| Antibody |
anti-DsbA(rabbit polyclonal) |
Beckwith lab |
- |
(1:1,000) in 5 w/v % skimmed milk/TBS-T |
| Antibody |
anti-AcrA(rabbit polyclonal) |
Koronakis lab |
- |
(1:10,000) in 5 w/v % skimmed milk/TBS-T |
| Antibody |
anti-TolC(rabbit polyclonal) |
Koronakis lab |
- |
(1:5,000) in 5 w/v % skimmed milk/TBS-T |
| Antibody |
anti-HtrA1 (DegP)(rabbit polyclonal) |
Abcam |
ab231195 |
(1:1,000) in 5 w/v % skimmed milk/TBS-T |
| Antibody |
anti-DnaK 8E2/2(mouse monoclonal) |
Enzo Life Sciences |
ADI-SPA-880-D |
(1:10,000) in 5% w/v skimmed milk/TBS-T |
| Antibody |
anti-rabbit IgG-AP conjugate (goat polyclonal) |
Sigma |
A3687-.25ML |
(1:6,000) in 5% w/v skimmed milk/TBS-T |
| Antibody |
anti-rabbit IgG-HRP conjugate (goat polyclonal) |
Sigma |
A0545-1ML |
(1:6,000) in 5% w/v skimmed milk/TBS-T |
| Antibody |
anti-mouse IgG-AP conjugate (goat polyclonal) |
Sigma |
A3688-.25ML |
(1:6,000) in 5% w/v skimmed milk/TBS-T |
| Antibody |
anti-mouse IgG-HRP conjugate (goat polyclonal) |
Sigma |
A4416-.5ML |
(1:6,000) in 5% w/v skimmed milk/TBS-T |
| Software, algorithm |
FlowJo |
Tree Star |
- |
version 10.0.6 |
| Software, algorithm |
Adobe Photoshop CS4 |
Adobe |
- |
extended version 11.0 |
| Software, algorithm |
Prism |
GraphPad |
- |
version 8.0.2 |
| Software, algorithm |
blastp |
Altschul et al., 1990
|
- |
version 2.2.28+ |
| Software, algorithm |
USEARCH |
Edgar, 2010
|
- |
version 7.0 |
| Software, algorithm |
MUSCLE |
Edgar, 2004
|
- |
- |
| Software, algorithm |
FastTree |
Price et al., 2010
|
- |
version 2.1.7 |
| Software, algorithm |
HMMER |
Finn et al., 2015
|
- |
version 3.1b2 |
